Resolucion 3099 de 2008 movies09.11.2019
Molecular genetic analysis of the nested Drosophila melanogaster lamin C gene. Fly lines used in this study are listed in Table 1. Pushing the envelope: structure, function, and dynamics of the nuclear periphery. Gradient of increasing affinity of importin beta for nucleoporins along the pathway of nuclear import. Asymmetric division of Drosophila neural progenitors. In particular, neuroblasts seem to undergo a semi-closed mitosis, similar to the mitosis reported previously for the embryonic tissues.
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Electrical properties of a-Ge-Se-In thin films. The average shot length of English language films has declined from had the most: A whopping 3, shots packed into minutes.
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In Vivo Dynamics of Drosophila Nuclear Envelope Components
Scholkopf, A. Smola, M. Tanaka, M. Okutomi, Super-resolution from image sequence under influence of hot-air optical turbulence.
Gradient of increasing affinity of importin beta for nucleoporins along the pathway of nuclear import.
The dynamics of GFP-Lamin C during mitosis, in particular its persistence in the spindle envelope until late metaphase, are consistent with earlier observations of lamin behavior based on microinjection of embryos with fluorescently labeled anti-lamin Fab fragments Paddy et al.
This article has been cited by other articles in PMC. Pushing the envelope: structure, function, and dynamics of the nuclear periphery. To further explore this issue, we compared the mitotic behavior of mRFP-Nup with that of GFP-tagged Mad2, which localizes to unattached kinetochores in mitosis Buffin et al.
Cdk1 and okadaic acid-sensitive phosphatases control assembly of nuclear pore complexes in Drosophila embryos.
Small regions outlined in white in the first frames within three or four nuclei of interphases 10, 11, or 12 were bleached.
Resolucion 3099 de 2008 movies
|Association of Mad2 with the NPCs at the end of mitosis is a two-step process.
The progressive nuclear accumulation of GFP-Mad2 begins once a full mRFP-Nup rim has formed open arrows and its subsequent enrichment at the NE only occurs several minutes later, during early G1 arrows. Megator, an essential coiled-coil protein that localizes to the putative spindle matrix during mitosis in Drosophila. Time-resolved, in vivo studies of mitotic spindle formation and nuclear lamina breakdown in Drosophila early embryos.
Here, we used an exon trap allele of ketel called ketel GFP in which a GFP exon is inserted between amino acids 18 and 19 of ketel within its Ran binding domain Morin et al. NPCs disassemble during prometaphase or even after metaphase in early C.
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Somewhat surprisingly, NE-associated Mad2 was only obvious in the later interphases cycles 12 and 13 of syncytial embryos. Cell Motil.
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As in syncytial embryos, mRFP-Nup staining could not be detected at kinetochores in control or colchicine-treated neuroblasts Figure 10 B; also see Figure 8 and Supplemental Movie 12indicating that the absence of detectable Nup at kinetochores is a general feature of fly mitosis.