Creb antibody cell signaling 919710888922.11.2019
Xing, J. The supernatant is the sample. Discard supernatant. Yin, J. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. Nuclease-free Water Reviews 4.
Wide species reactivity, highly cited and reviewed CREB antibodies. Monoclonal Antibody for studying CREB in the Neuroscience research area.
Polyclonal Antibody for studying CREB in the Neuroscience research area.
Lonze, B. Entrez-Gene Id: NOTE : Adherent cells or tissue should be dissociated and in single-cell suspension prior to fixation. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations Proceed to immunoprecipitation section.
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Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available.
CST CREB (D76D11) Rabbit mAb
CREB is able to mediate signals from numerous physiological stimuli, resulting in Anti-rabbit secondary antibodies must be used to detect this antibody.
Ribar, T. NOTE : Cells should be grown, treated, fixed and stained directly in multi-well plates, chamber slides or on coverslips.
All reagents required for this protocol may be efficiently purchased together in our Intracellular Flow Cytometry Kit Methanolor individually using the catalog numbers listed below. Normal Goat Serum Reviews 4.
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Dilute to 1X with dH 2 O. This product has been approved for use in this application by CST. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system.
Keep on ice. Do not aliquot the antibody. Save This Selection.
Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity. Pellet cells by centrifugation and remove supernatant. Immunofluorescence Blocking Buffer Reviews 4. Incubate specimen in fluorochrome-conjugated secondary antibody diluted in Antibody Dilution Buffer for 1—2 hr at room temperature in the dark.